In the process of modern planting, adzuki bean has encountered some problems, its degree of quality diversification is relatively low, and its biological and non-biological stress capacity is relatively weak, which greatly limits the development of related industries. Therefore, the use of existing germplasm resources to select high quality varieties is an urgent issue at present. For the adzuki bean crop, there is currently no effective in vitro culture system for plant regeneration and genetic transformation, which limits the application of transgenic technology in genetic improvement of adzuki bean crops. The tissue culture chamber plays an important role in the study of genetic improvement and provides a growth environment for the development of related tissues.
Adventitious bud induction rate of adzuki bean was higher, the induction rate was 76.4%, and the average number of buds was 6.2. It was an ideal explant. About 20 days later, a small greenish, vigorously growing tissue was grown on the cut portion of the adaxial axis of adzuki bean, and adventitious buds were grown 42 days later. However, adventitious buds were not induced in the explants of Adzuki Bean Leaves, browning occurred after inoculation, and with the prolongation of subculture time, browning became more and more serious and eventually died, and adventitious buds could not be obtained.
From the tissue culture chamber study, it was found that 6-BA concentration had a significant effect on the induction of adzuki bean explants, but when the concentration was higher, the shoots appeared vitrification, while the addition of GA3 could significantly reduce the rate of glass seedlings, but When the concentration is greater than 0.5 mg/L, the growth of buds is adversely affected. The type and concentration of auxin have important effects on adventitious bud elongation and root formation in vitro. 0.1 mg/L IA is suitable for the adventitious bud growth of adzuki bean; 1.0 mg/L NAA is suitable for the rooting of adzuki bean test tube seedlings, and the regeneration is complete. Plants have beautiful shape, dark green color, and well-developed root systems. They grow well after transplanting.
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